Comparison of RNA Integrity in Paraffin, Cryo and PEG Embedded Specimen
Agilent Bioanalyzer electropherograms display RNA obtained from tissue sections processed for EFIC imaging. The presence of 28S, 18S and 5S rRNA peaks indicated good RNA integrity from formalin fixed-frozen, formalin fixed-PEG, and unfixed frozen embedded specimen. In contrast, RNA profile from the formalin fixed-paraffin embedded samples indicate severe RNA degradation.